RT-qPCR检测 z-bo 2020-12-10 354

　　Objective To establish a real-time fluorescence quantitative (RT-qPCR) method to detect the transcription level of the xanthine hydrogenase/oxidase (XDH/XO) gene in Wistar rats to quantitatively detect the XDH/XO gene at the transcription level. Methods Total RNA from the liver tissue of Wistar rats was extracted, cDNA was obtained by reverse transcription, diluted to 5 concentration gradients with a dilution factor of 10 times, and RT-qPCR detection was performed using the designed primer sequence and internal reference gene to obtain XDH/XO gene expression The standard curve. Then detect the changes of XDH/XO gene transcription level in Wistar rat hyperuricemia animal model.

　　 Results The standard curve of XDH/XO gene detected by RT-pPCR method has a single dissolution peak and R2 is close to 1, which can detect the change of XDH/XO gene transcription level in the animal model of hyperuric acid.

　　 Conclusion The RT-qPCR method for detecting the transcription level of XDH/XO genes in Wisar rats has the characteristics of quantitative accuracy and good reproducibility, and can be applied to the pathogenesis of hyperuricemia and new drug research.