CRT,SD大鼠 z-bo 2020-12-10 355

　　Objective To establish a rat external jugular vein catheter-related thrombosis (CRT) model, observe the formation of rat CRT, and detect tissue factor (TF), thrombomodulin (TM) and von Willebrand disease in rat serum The content of factor (vWF), study its relationship with the formation of CRT. Methods 120 male SD rats were randomly divided into a blank control group (n = 40), a sham operation group (n = 40), and a model group (n = 40). Each group was performed on the 1st, 4th, and 4th day after surgery. Eight rats were randomly selected from 7 days, 10 days, and 14 days, and pathological sections were used to observe the thrombosis of the rats. Serum TF, TM, and vWF levels were detected by ELISA.

　　 Results Neither the blank control group nor the sham operation group had thrombosis. There were 34 CRTs in the model group, and the incidence of CRT was 85%. The TM content of CRT rats in the model group was significantly higher than that of rats without CRT (P<0.01), and the TM content of rats without CRT in the model group was significantly higher than that of the sham operation group (P<0.01) The content of TM and vWF in the model group without CRT was significantly higher than that in the blank control group (P<0.01). At various time points after operation, the contents of TF and TM in the model group were significantly higher than those in the blank control group and the sham operation group (P<0.01). On the 4th, 7th, 10th, and 14th day after operation, the vWF content of the model group was significantly higher than that of the blank control group and the sham operation group (P<0.01). As the observation time prolonged in the model group, the serum TF, TM, and vWF contents gradually increased with time. Among them, the TF and vWF contents increased sharply from 1 to 4 days after the operation, reached the peak 10 days after the operation, and then dropped sharply. ; TM content increased the most from 4 to 7 days after surgery, reached a peak at 7 days after surgery, and then began to decrease.

　　 Conclusion The formation of CRT in rats is closely related to the abnormal expression of TF, TM and vWF in serum.