动物造模 z-bo 2022-07-07 431

　　[Model mechanism] Arrhythmia caused by aconitine is mainly related to inhibiting membrane Na + channel inactivation, promoting continuous Na + influx and improving the autonomy of ectopic pacemakers. In addition, aconitine can significantly increase the influx of Ca 2+. It inhibits K + efflux, prolongs the repolarization time, significantly increases the incidence of repolarization, causes reentry excitement, and forms single-gene or multi-source ectopic heart rhythm. The release of catecholamines in large doses can be Activate sympathetic nerves and promote internal. [Modeling method] Models of arrhythmia caused by a single injection of aconitine from various animals such as mice, rats, rabbits and guinea pigs. After the animal is anesthetized, the animal is fixed in the dorsal position, inserted into the skin, and lead II ECG is recorded, and the ECG changes of the animal are continuously and dynamically observed through the ECG oscilloscope. After recording a part of the animal's normal ECG, a rapid sublingual intravenous injection of 40μg/kg (injection is completed within 3-5 seconds), most animals develop arrhythmia within 4-5 minutes. development of.

　　[Model Features] The model has a simple method, fast response speed, long duration, and is sensitive to class I antiarrhythmic drugs.

　　[Model Evaluation and Application] can be used to screen anti-arrhythmic drugs.