红系祖细胞 z-bo 2020-12-11 370

　　Purpose: To evaluate the transplantation effect of erythroid progenitor cells in cloche-/- mutant zebrafish with congenital primitive hematopoietic defects. Method: Collect the zTg (gata1:EGFP) zebrafish embryos of the transgenic line with green fluorescently labeled erythroid progenitor cells, prepare them into a single-cell suspension, sort out the gata1+ cells with green fluorescence by flow cytometry, and use microinjection technology Transplant gata1+ cells into 42hpf cloche-/- mutant zebrafish heart, and use stereo fluorescence microscope to track the expression of erythroid progenitor cells in cloche-/- mutant zebrafish after transplantation.

　　Result: The gata1+ cells with green fluorescence were successfully sorted out, and the gata1+ cells with green fluorescence were observed to proliferate and diffuse gradually at 2h after transplantation, and the green fluorescence expression continued for 16h.

　　 Conclusion: Erythroid progenitor cells have the potential to rebuild blood, which provides experimental basis for further research on the functional identification of erythroid progenitor cells after transplantation.