基因组 z-bo 2020-12-11 354

　　A few days ago, with the support of the Shandong Agricultural Good Species Project and other projects, the team of researcher Chen Songlin of the Yellow Sea Fisheries Research Institute, the team of Academician Lin Haoran of Sun Yat-sen University and the team of Professor Li Weiming of Michigan State University in the United States jointly completed the grouper (Gentiana) The whole genome sequencing and genome mapping of the company, and analyzed its innate immunity and rapid growth genomics mechanism. The research results are published online in the important international academic journal "Molecular Ecology Resources".

　　 Grouper is an important marine cultured economic fish in my country. Saddleback grouper, commonly known as gentian grouper and dragon scorpion, is the largest and fastest growing grouper among groupers. It has the characteristics of strong disease resistance, fast growth and delicious taste, so it is widely used Yu grouper hybrid breeding and elite breed selection. As the largest carnivorous rock fish, saddlefish also has an important influence on the rocky ecosystem.

　　Researchers used next-generation sequencing and HiC three-dimensional genome technology to construct a chromosome-level grouper grouper genome map. The results showed that the grouper grouper genome size is 1.086Gb and contains 24718 protein-coding genes. Evolutionary analysis and collinearity analysis show that the relationship between grouper saddlefish and three spines spines is the closest, and the differentiation time is about 100 million years ago. Some chromosomes of the two have merged or split; comparative genomics analysis with other fish genomes It is found that the grouper has a relatively complete innate immune system. Some of the gene families involved in innate immunity (such as the NOD-like family) have undergone significant expansion and significant changes in DNA sequence (positive selection); Some key genes of hormone signaling pathways are also subject to positive selection, which may be related to the biological characteristics of rapid growth. According to reports, the completion of the fine map of the grouper's genome has laid an important foundation for the analysis of the molecular mechanisms of important economic traits such as growth and disease resistance of the grouper. At the same time, the whole genome data of the grouper is also useful. The development of grouper hybrid breeding and improved breed selection technology provides valuable genetic resources, which is of great significance for promoting the sustainable development of grouper industry.