动物造模 z-bo 2022-02-03 446

　　[Model mechanism] Myocardial ischemia is a state in which the supply of the heart decreases when the blood perfusion of the heart decreases, and the energy metabolism of the myocardium is abnormal and normal heart function cannot be maintained. Various arrhythmias may occur centered on arrhythmia. These mechanisms include abnormal autonomy, reentry and trigger activities. In the early stage of ischemia (within 10 minutes of coronary artery ligation), ventricular arrhythmia may be caused by reentry stimulation, and abnormal pacing activity may occur between 15 and 20 minutes. [Modeling method] This model has been successfully replicated in mice, rats, rabbits, dogs, pigs and other animals. After anesthetizing the animal, fix it in the supine position, intubate the trachea, and then connect the ventilator to the electrocardiograph. Prepare the skin, open the chest in the fourth or fifth intercostal space on the left, cut open the pocket of the heart, and then gently press the right chest to press out the heart. The anterior descending branch of the left coronary artery was separated and ligated, and the heart was returned to the chest cavity. At the same time, the ST segment was significantly elevated through ECG monitoring, and the operation was successful.

　　[Model Features] This model is easy to operate, and the spontaneous incidence of arrhythmia is about 50%. It is mainly paroxysmal ventricular tachycardia, which can be combined with procedural electrical stimulation to cause ventricular fibrillation.

　　[Model Evaluation and Application] is used to study the mechanism and treatment of coronary heart disease arrhythmia.