Hif-α基因 z-bo 2020-12-11 337

　　Objective: To explore the role and expression regulation of hypoxia-inducible factor Hif-α in the adaptation to hypoxic environment of Schizomerinae.

　　Methods: Using RT-PCR technology to obtain the Hif-1αΑ, Hif-1αB, Hif-2αΑ and Hif-2αB gene coding region sequences of 13 representative species of Schizoma subfamily fishes, based on the Schizoma subfamily fish system Evolutionary relationship has carried out gene selection pressure analysis, and selected naked carp for hypoxia-induced expression research. Results: The coding regions of Hif1αΑ, Hif-1αB, Hif-2αΑ and Hif-2αB genes were 2196, 2325, 2544, and 2511 bp in length, respectively. The sequence alignment revealed that the proline hydroxyl group in the HIF1αΑNODD domain of the subfamily fish of the schizomeridae The motif LxxLAP is missing, and the proline hydroxylation motif in the CODD domain of HIF1αB in the specialized and highly specialized split-bellied fish is mutated to PxxLAP. Compared with the normoxic group, the piebald is bare under severe hypoxia. The expression of some Hif-α genes in the brain and heart tissues of carp significantly decreased, and the expression of Hif-1αA and Hif-2αA genes in the heart tissues was up-regulated in moderate hypoxia.

　　Conclusion: Hif-α gene is subject to positive selection in 13 species, but specific species branches need to be further studied. At the same time, Schizomerinae may have two different expressions in adapting to chronic hypoxia and acute hypoxia. Regulation mechanism.