【Animal Modeling】-Pituitrin-induced angina pectoris animal model

动物造模

z-bo

2022-10-06

611

[Model mechanism]: Pituitary prostaglandin strongly constricts the coronary arteries, causing acute failure of blood supply to myocardial tissue, increasing peripheral resistance, increasing the burden on the heart, causing myocardial ischemia, hypoxia and even necrosis.

　　[Modeling method]: Place the animal on the supine position of the interventional operating table and connect it to the ECG machine (exclude animals with abnormal ECG). Pituitary vasopressin was injected intravenously into the ear edge with a dose of 1.5-2.5 U/kg. After the injection, the injection was completed within 30 seconds, 0 seconds, 5 seconds, 10 seconds, 15 seconds, and 30 seconds. Record 1 minute, 2 ECG minutes, 5 minutes, 15 minutes, 20 minutes. After successful modeling, the rabbit ECG will be divided into two stages. The first stage is within 30 seconds after injection. The symptoms are: T wave rises, ST segment rises (0.1 mV or higher), the most obvious in the last 10 seconds. The phase performance is after 30 seconds. The T wave is low, flat, two-way or inverted, and there is no obvious change in the ST segment, thereby extending P? R and Q? The T phase can last for several minutes to tens of minutes. This specific ECG change is not only a sign of the success of the model, but also a criterion for evaluating intervention factors such as drugs. In model animals, the ability to prevent or reduce biphasic changes is considered therapeutic.

　　 Rat: Choose about 250 grams of Wistar rat. Anesthetized by intraperitoneal injection of urethane, fixed in the supine position, inserted electrode needles into the skin of the limbs and chest, and connected to the electrocardiograph for recording. Pituitrin was injected into the sublingual or tail vein at a dose of 0.5 or 1.0 U/kg. The injection is usually completed within 5 or 10 seconds. Judge the model effect and intervention effect according to the ECG changes of the rabbit model. [Model Features] The model method is stable and reproducible, relatively easy to operate, does not require thoracotomy, and has low animal mortality. However, coronary artery spasm caused by pituitrin injection is non-specific, and there are certain differences in the onset of clinical patients.

　　[Evaluation and Application of Model]: This model is used to study angina pectoris, especially for the treatment of coronary artery spasm and coronary artery dilation. Pituitrin-induced myocardial selectivity is relatively low, and coronary artery contraction is not specific. The degree and dose of myocardial ischemia in animals vary greatly among animals. When choosing this model, you should pay attention to these issues.