动物造模 z-bo 2022-04-22 476

　　[Modeling mechanism]: Isoproterenol is a catecholamine-based drug, which is a β-adrenergic receptor agonist, which can increase myocardial contraction and oxygen consumption, thereby increasing myocardial burden and myocardial microcirculation, and Causes myocardial damage.

　　[Modeling method]: Choose about 250 g Wistar rats, inject intraperitoneally with carbamate for anesthesia, fix them in the supine position, insert electrode needles into the skin of the limbs and chest, and connect to the electrocardiograph for recording. Starting from the first day of 0, 5, 10, 15, and 30 injections, the dose of subcutaneous injection of isoproterenol is 2-8 mg/kg (10-16 mg/kg for rabbits), once a day for 2 consecutive days Record ECG within 45 minutes, 60 minutes within 30 minutes, ECG 24 days or 48 hours after administration. After injection of isoproterenol, ECG may be related to T wave inversion or biphasic ST-segment elevation with sinus tachycardia, premature contraction or other arrhythmia. 48 hours after the injection, the heart of the model animal was taken out for pathological examination. Local necrosis under the microscope was observed in the myocardium, and the infiltration of inflammatory cells and macrophages was observed under the microscope.

　　[Model Features]: Compared with pituitary necrosis myocardial necrosis, it is easy to create, highly replicable and more stable. The pathological characteristics of myocardial necrosis are similar to those caused by human myocardial ischemia and hypoxia, but the etiology is still slightly different from humans.

　　[Model Evaluation and Application]: This model is suitable for observing the balance between myocardial ischemic necrosis and myocardial oxygen supply, and is also suitable for evaluating and screening β-adrenergic receptor-related drugs. Because the dose of isoproterenol is relatively large, care should be taken when modeling animals.