艾滋病毒 z-bo 2020-12-17 352

　　Recently, the reporter learned from the Wuhan Institute of Virology of the Chinese Academy of Sciences that the Cui Zongqiang research team and the Zhang Xianen team of the Institute of Biophysics of the Chinese Academy of Sciences have dynamically observed the uncoating process of a single HIV in real time, revealing that the matrix protein, capsid protein, The dissociation process and spatiotemporal mechanism of different levels and different components of virus nucleic acid and so on.

　　 Cui Zongqiang’s team first established a real-time tracing analysis method for HIV multicolor fluorescent labeling and single virus in living cells to dynamically and visually track the virus unpacking process. Using metal ruthenium organic complexes, double arsenic dyes and fluorescent proteins, the viral genome RNA, capsid protein CA and matrix protein MA were fluorescently labeled, and two-color and three-color fluorescently labeled virus particles with good infection ability were constructed. The research team combined with the single particle tracing technology in living cells to conduct real-time, dynamic and visual analysis of the dissociation and uncoating process of a single HIV invading host cells, revealing that HIV infects host cells within 60 to 120 minutes , The viral genome, capsid protein and matrix protein dissociate in a dynamic sequence similar to a "rocket lift-off step by step separation" pattern. Based on drug inhibition and statistical analysis, scientists have found that the time and efficiency of the virus dissociation and uncoating process will be affected by the cytokine CypA and the virus reverse transcription process.

　　This research is the first real-time dynamic analysis of the uncoating process of a single HIV in living cells in the world. It is of great significance for in-depth understanding of the life cycle of HIV in host cells, and will also provide ideas for the development of new antiviral pathways.