细菌耐药基因组 z-bo 2020-12-18 297

　　The latest progress of Zhu Baoli's research group in the genomics research of bacterial resistance of the Institute of Microbiology, Chinese Academy of Sciences. The research is based on the big data of genomics for the first time. It has deeply analyzed the transmission network and law of drug resistance genes between bacteria, and has a deep understanding of bacterial resistance. The evolution of bacteria and the formation mechanism of bacterial resistance are of great significance.

　　 Bacterial resistance is an important challenge facing global public health. In recent years, with the continuous emergence of "super bacteria" carrying drug-resistant genes such as NDM-1 and MCR-1, the problem has become increasingly prominent. Bacteria have multiple resistance mechanisms, which are encoded by a large number of resistance genes. However, drug resistance genes, especially those with high-risk mobility, have not fully revealed the transmission network and driving force between bacteria and human and animal intestinal flora. Recently, the research team Hu Yongfei and others have conducted research on more than 23,000 known bacterial genomes, 9.8 million published human gut bacterial genes, and sequenced 300,000 cultured animal gut bacterial genes with high-risk mobile resistance genes. A comprehensive analysis.

　　 This study found that mobile drug resistance genes mainly exist in 790 bacterial species among the 4 bacterial phyla, and their abundance and transfer frequency are significantly enriched in the Proteobacteria. The recent transfer of these resistance genes between bacteria has formed a huge network consisting of 703 bacterial species and 16859 interspecies pairings. Analyzing the network, it is found that the transfer of drug resistance genes between bacterial individuals is dominated by the evolutionary relationship of bacterial species and is also subject to ecological barriers; this rule is also applicable to drug resistance genes at the level of human and animal intestinal bacterial populations Transfer. Further analysis revealed that 33 of 41 drug resistance genes that transfer between human and animal intestinal flora are present in a variety of human pathogenic bacteria.