PC12细胞氧化应激和凋亡 z-bo 2020-12-18 311

　　Objective To investigate the effect of betulin on oxidative stress and apoptosis of PC12 cells induced by Aβ25-35. Methods 40 μmol/L Aβ25-35 was applied to PC12 cells. The experiment was randomly divided into control group, Aβ25-35 group, N-acetylcysteine group (NAC group) and betulin 5, 10, 20 μmol/L groups. MTT method was used to detect cell survival rate, Annexin V-FITC/PI double staining flow cytometry was used to detect apoptosis, JC-1 kit was used to detect mitochondrial membrane potential, LDH, MDA, and SOD kits were used to detect LDH, MDA and SOD, respectively Level, colorimetric method to detect the enzymatic activity of caspase3, caspase8, caspase9 and Cyt c, Western blot method to detect the activity and expression of apoptosis-related proteins Bcl2, Bax, caspase3, caspase8, caspase9 and Cyt c. Results Compared with the Aβ25-35 group, both betulin and NAC can increase the cell survival rate and SOD activity (P<0.01) of PC12 cells induced by Aβ25-35, and reduce the LDH release rate and apoptosis rate (P<0 01), inhibit caspase3, caspase8, caspase9 and Cyt c enzyme activity (P<0.01), while down-regulating the expression levels of Bax, caspase3, caspase8, caspase9 and Cyt c (P<0.01), and up-regulate the activity of Bcl2 And expression (P<0.01).

　　 Conclusion By preventing and giving effective doses of betulin can inhibit Aβ25-35-induced cell apoptosis, the mechanism may be related to the reduction of ROS production, thereby reducing the level of oxidative stress, and then inhibiting the pathway of cell apoptosis.