小鼠原发性肝癌建模 z-bo 2020-12-22 279

　　Objective: To establish a good, fast and stable mouse model of primary liver cancer.

　　Methods: 128 male KM mice were randomly divided into 8 groups: the normal group was given free diet; the diethylnitrosamine (DEN) aqueous solution group was given an aqueous solution containing DEN (16.5 mg/kg) daily; high-dose DEN sesame oil Group (7 times/week, 3 times/week, 1 time/week), oral administration of a sesame oil solution containing DEN (16.5 mg/kg); while the low-dose DEN sesame oil group, nano-DEN group, and nanocarrier group were given respectively Every week, sesame oil solution containing DEN (8.25 mg/kg) and nano-DEN or the same amount of nanocarrier were given orally every week. After 20 weeks, the mice were sacrificed, liver samples were taken, and HE staining was performed after fixation.

　　Results: (1) The mice in each group containing DEN had different degrees of dull coat color, dullness, slow movement, and slow weight gain; (2) At 20 weeks, the mice in the same DEN water solution group and high-dose DEN sesame oil group appeared Tumors, and the mortality rate of mice at 1 time/week was low; (3) No deaths occurred in the mice in the Nano-DEN group, and the HE score of liver damage was severe in the lower-dose DEN sesame oil group.

　　Conclusion: Oral administration of DEN dissolved in sesame oil increases the compliance of mice with medication. Long-term low-frequency administration can greatly reduce the mortality during the modeling process. The nano-microemulsion preparation of DEN can enhance liver damage degree.