复合刺激创伤后应激障碍模型 z-bo 2021-11-11 365

　　Objective: To study the pathological changes of important brain areas in rats with post-traumatic stress disorder after combined stimulation.

　　Methods: Twenty adult SD female rats were randomly divided into normal group and model group, with 10 rats in each group. The PTSD rat model was established in the model group according to the compound stimulation method. Four weeks later, the elevated plus maze and Morris were performed on the two groups of rats Water maze test; After the behavior test, HE staining and Nissl staining were performed on the rat cerebral cortex, hippocampal CA1, CA2, CA3 area and dentate gyrus to observe the characteristics of pathological changes.

　　Results: In the normal group, cells in the cerebral cortex and hippocampus were regular and evenly distributed, with clear nucleoli and borders, abundant cytoplasmic Nissl bodies, and no obvious neuronal degeneration and necrosis; the cerebral cortex cells in the model group were relatively regular and evenly distributed, without obvious Pathological changes; cells in the CA1 and CA3 areas of the hippocampus are irregular in shape and arranged disorderly, the intercellular space is enlarged, the number of cells is reduced, there are more vacuole-like cytopathies, and the CA3 area is more obvious; the cells in the CA2 area are arranged more regularly and distributed more uniformly In the dentate gyrus, some cells are loosely arranged, intercellular spaces are enlarged, and the number of Nissl bodies is reduced.

　　Conclusion: PTSD model rats have different degrees of pathological changes in hippocampus CA1, CA3 and dentate gyrus, which provides an experimental basis for exploring the pathological mechanism of PTSD patients.