慢性输卵管炎症模型 z-bo 2020-12-22 316

　　Objective To establish a model of chronic inflammation of the fallopian tubes in New Zealand rabbits using Escherichia coli.

　　Method Prepare 3×109/mL mixed bacteria liquid (Escherichia coli: Staphylococcus aureus: Streptococcus mixed in 2:1:1 volume) and 3×108/mL Escherichia coli suspension. Eighteen New Zealand female rabbits were randomly divided into three groups, including 2 in the normal group, 8 in the mixed bacterial liquid experimental group, and 8 in the Escherichia coli liquid experimental group. The mixed bacterial liquid and Escherichia coli liquid were injected into the two groups of experimental rabbits by transvaginal intubation. The gross specimens and the formation of fallopian tube inflammation under light microscope were observed on the 5th, 10th, 15th, and 20th days after intrauterine perfusion with bacterial liquid.

　　Result The rabbits in the mixed bacteria liquid group and Escherichia coli group showed chronic fallopian tube inflammation on the 15th day of the perfusion.

　　 Conclusion Both modeling methods have the advantages of being close to the human infection pathway, high modeling rate, and simple operation. They are effective methods to establish animal models of chronic inflammation of the fallopian tube.