肺腺癌,裸鼠模型 z-bo 2020-12-22 338

　　Objective: To establish a model of lung adenocarcinoma A549 subcutaneously inoculated in green fluorescent nude mice to study the source of vascular endothelial cells during the angiogenesis of lung adenocarcinoma. Methods: A nude mouse model of lung adenocarcinoma A549 was subcutaneously inoculated with GFP, and CD 31 was stained by immunofluorescence staining method to label tumor blood vessels; tumor tissues were observed and photographed frozen sections by fluorescence microscope; tumor tissue endothelium was detected by immunohistochemistry GFP expression in the cell.

　　 Results: The results of immunofluorescence experiments showed that: GFP protein and CD 31 protein can be co-expressed in some interstitial blood vessels; some blood vessels express CD 31 protein but not GFP protein. The results of immunohistochemistry experiments: the cytoplasm of some mesenchymal cells and endothelial cells in tumor tissues express GFP protein.

　　 Conclusion: The endothelial cells that make up tumor neovascularization are partly derived from nude mice and partly derived from tumor cells.