妊娠期糖尿病小鼠模型 z-bo 2020-12-22 331

　　Objective: To explore a stable and reliable method for establishing a mouse model similar to insulin resistance during pregnancy.

　　Methods: 60 SPF 5-week-old KM mice were randomly divided into a high-fat diet group and a general diet group. The high-fat diet group was fed with high-fat diet for 4 weeks and then caged with 1:1 male and female, and viewed in the vagina of female mice. To the first day of pregnancy positioning the vaginal suppository. After successful pregnancy, intraperitoneal injections were performed at 30 mg/kg intervals for 24 hours, a total of 3 injections. The control group was injected with the same amount of citrate buffer (0.1 mol/L, pH=4.2). On the 3rd, 7th, 14th and 19th days after the successful modelling, the random blood glucose, body weight, and 24-hour water intake and food intake of the mice were recorded. ELISA detects the concentration of INS, ADP, LEP and CRP factors in serum.

　　Results: After the successful modeling, pregnant rats showed obvious symptoms of increased water intake, food intake, and urine output. Compared with the control group, the water intake and food intake were significantly different (P<0.01), and="" the="" model="" blood="" glucose="">11.1 mmol/L , Significantly higher than the control group. In the GDM group, INS (1.50±0.25) Mu/L, ADP (0.65±0.13) μg/L, LEP (1.60±0.12) μg/L, CRP (37.54±4.70) μg/L, the difference is extremely significant compared with the control group (P<0.01). After giving birth, the blood glucose of the mice returned to normal levels.

　　Conclusion: High-fat diet combined with low-dose STZ multiple induction methods can establish a GDM model and conform to the characteristics of pathological insulin resistance in human gestational diabetes.