小鼠流感病毒气溶胶感染模型 z-bo 2020-12-22 322

　　Purpose: To infect three strains of mice separately through aerosol attack to produce aerosol, and compare the infection differences of the three strains of mice during the infection process, so as to analyze the characteristics of the three strains of mouse influenza models , To provide reference for the research of influenza pathogenesis and the development of vaccines and drugs to select suitable infection models.

　　Methods: A/Puerto Rico/8/34 (H1N1) virus strain was selected, and aerosol challenge was used to infect mice of three strains of inbred lines C57BL/6, BALB/c and outbred group ICR, weighed daily The body weight of the mice was observed by naked eyes. The mice were sacrificed at 3, 7, and 14 days after infection, and the lungs were taken to weigh their wet weight, and the lung virus was determined and pathologically observed.

　　Results: All three strains of mice can be infected. Among them, the survival rate of C57BL/6 mice is lower than that of the other two strains. The lung index and viral load of 3 days are significantly higher than that of ICR mice (P<0.05). The following pathological results show that it is more obvious than the other two strains. Compared with the other two strains of mice, BALB/c mice have the slowest body weight recovery in the later stage, and the survival rate is higher than C57BL/6 but lower than ICR; lung index and viral load are no different from those of the other two strains. Significance; the pathological changes under microscope are similar, but weaker than C57BL/6 and stronger than ICR. The pathogenesis of ICR mice is basically similar to that of the other two strains, but the indicators of body weight, survival rate, lung index, viral load and pathological changes under microscope are weaker than those of the other two strains.

　　Conclusion: Three strains of mice can establish influenza aerosol models, but the three models after infection have their own characteristics. In the experiment, suitable mouse strains can be selected to establish models according to different research purposes.