哮喘模型 z-bo 2020-12-23 305

　　Objective: To observe the effect of paeoniflorin on airway inflammation chemokines and receptors in a mouse asthma model. Methods: A mouse asthma model was established with ovalbumin (OVA) sensitization and challenge; serum IL-6, TNF-α levels and bronchoalveolar lavage fluid (BALF) were detected by ELISA method for egg protein-specific IgE (OVA) -IgE) and chemokine CCL19, CCL21 levels; RT-PCR method to detect chemokine receptor CCR7mRNA expression in lung tissue; Western blot to detect lung tissue CCR7 and nuclear factor-κB (nuclear factor-κB, NF-κB) Protein.

　　 Results: The levels of serum IL-6 and TNF-α in mice in the paeoniflorin intervention group were significantly decreased; the levels of OVA-IgE, CCL19 and CCL21 in BALF were significantly decreased; the expression of CCR7mRNA, CCR7 and NF-κB in lung tissue was significantly decreased.

　　 Conclusion: Paeoniflorin has a significant inhibitory effect on the airway inflammation chemokine CCL19/CCL21 and its receptor CCR7 in asthma model mice.