动物造模 z-bo 2022-02-02 443

　　[Modeling mechanism] Monocrotaline (MCT) is a plant extract of the genus Crotalaria. It is a double-layer pyrrolidine alkaloid that can carbonize the DNA of cancer cells and exert anti-cancer effects, mainly after injection in the body. Will be used. It is distributed in the liver, kidneys and stomach. Monocrotaline is widely used to replicate pulmonary circulation hypertension models. Monocrotaline is activated by the liver and becomes electrophilic monocrotaline, which damages the pulmonary vascular endothelium and subsequently causes pulmonary vascular remodeling. Monocrotaline can repeatedly cause severe progressive pulmonary hypertension. Commonly used laboratory animals include mice, rats, rabbits and miniature pigs.

　　[Modeling method] Male Wistar rats. Single crocrotaline crystals were made into 2% solution and injected intraperitoneally at 60 mg/kg. Animals were sacrificed 14, 21 and 28 days after injection. Perform right heart catheterization, measure the pressure of the right ventricle and pulmonary artery, and perform cardiopulmonary pathology.

　　[Characteristics of the model] Pulmonary artery pressure increased three-fold, and right ventricular hypertrophy and weight loss in rats caused optical vessels to be re-examined under an optical microscope showing intimal hyperplasia, thickening of the intermediate layer and tissue. You can model. [Model Evaluation and Application] Currently, the atmospheric hypoxic pulmonary hypertension model is used because it has a similar incidence with simple pulmonary heart disease.