解脲支原体感染动物模型 z-bo 2021-11-01 307

　　Objective: To explore the advantages and disadvantages of different modeling methods, and to select the most suitable animal model method for Ureaplasma urealyticum infection. Methods: SPF-grade BALB/c female mice and Wistar rats were selected, and the intervention measures were multiple infections with low-dose UU3 bacterial solution, one infection with high-dose UU3 bacterial solution, and estradiol benzoate pretreatment as intervention measures to establish Ureaplasma urealyticum Infected animal models, the cervical secretions of the experimental animals were taken 14 days, 28 days, 42 days, and 56 days after the first vaccination for Ureaplasma culture, and the genitals were taken for morphological analysis.

　　Results: ①Compared with Wistar rats, BALB/c mice have a worse general condition, increased vaginal secretions, and obvious vulvar ulceration, while Wistar rats have no obvious changes in vulva. ②Comparison of vaginal UU colonization rate in each group of rats was statistically significant (P<0.05); compared with low-dose estrogen group and low-dose non-estrogen group, the difference was statistically significant (P<0.05); high-dose The difference between the estrogen group and the high-dose non-estrogen group was statistically significant (P<0.05); the difference between the low-dose estrogen group and the high-dose estrogen group was statistically significant (P<0.05); There was a statistically significant difference between the estrogen group and the high-dose non-estrogen group (P<0.05). The results of each group of mice were the same as those of rats. ③The same intervention measures, under the treatment of low-dose estrogen, the difference was statistically significant between rats and mice (P<0.05); under the treatment of high-dose estrogen, the difference was statistically significant between rats and mice ( P<0.05), indicating that the vaginal UU colonization rate of mice is higher under the same treatment measures. ④ Macroscopic view: Individual rats in the low-dose estrogen group have edema in the reproductive tract tissues, while the other groups have soft reproductive tract tissues and good mobility; the control group has soft reproductive tract tissues and good mobility; low-dose estrogen group The reproductive tract tissues of mice were hyperemia, hydrosalpinx, thickened, stiff, and poor elasticity; the mice in the high-dose estrogen group were mainly loose and edema; the low-dose non-estrogen group and the high-dose non-estrogen group only saw the vagina and cervix Mild congestion and edema. ⑤Microscopic view: There was no obvious pathological damage to the vaginal tissue of the rats in each intervention group, and some loose tissue edema was seen. The mice in the low-dose estrogen group suffered severe pathological damage; the low-dose non-estrogen mouse group and the high-dose estrogen mouse group had less congestion and edema, slightly dilated glands, and more inflammatory secretions.

　　Conclusion: Compared with Wistar rats, BALB/c mice have obvious vulvar damage; estrogen pretreatment can increase the colonization rate of U. urealyticum in experimental animals; low-dose multiple infections can increase the UU rating rate more than high-dose single infection ; UU colonization rate is higher in the vagina of BALB/c mice; BALB/c mice have severe pathological damage, which is of greater value for animal experiments.