肝纤维化模型 z-bo 2020-12-23 335

　　Objective: To verify whether iron can accelerate the process of liver fibrosis in rats.

　　Method: Rats were divided into control group, high-fat group, high-iron group, high-fat and high-iron group, and high-fat deironing group, with 24 rats in each group. While freely ingesting normal diet and high-fat diet, rats in the high-iron group and high-fat high-iron group were intramuscularly injected with iron dextran 50 mg/kg every other day; Ferric ammonium 30 mg/kg, 3 times/week; 8 rats were selected in the 4th, 5th, and 6th months of the intervention, and the hyaluronic acid (HA) and collagen type IV (COL-IV) were tested. , Laminin (LN), procollagen Ⅲ (PC Ⅲ); Masson staining to observe liver pathological changes. Results: HA levels in the high-fat and high-iron group at the fifth month of intervention were higher than those in the control and high-fat groups; at the sixth month, the levels of COL-IV and LN in the high-fat and high-iron group were higher than those in the high-fat group. Serum PC Ⅲ level reached 1.63 times of the high-fat diet group. Masson staining of liver specimens in the high-fat and high-iron group showed significant collagen deposition, but no collagen deposition in other groups.

　　 Conclusion: Iron can accelerate the process of liver fibrosis in rats caused by high fat.