凝血酶法致大鼠血液高凝态模型 z-bo 2020-12-23 324

　　Objective: To explore the rat model of hypercoagulability caused by intravenous injection of thrombin, and to provide a suitable animal model for the study of hypercoagulability.

　　Methods: SD rats were divided into six groups. Normal saline and 2.5, 5, 10, 20, 40 U/kg thrombin solution were injected into the femoral vein at a constant rate. Blood was collected within 5 minutes to measure the activated partial thromboplastin time (activated partial thromboplastin time). Thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), and observe the death of rats to determine the optimal thrombin dose. On this basis, the optimal dose of thrombin solution was injected into the femoral vein, and blood was collected at 0, 10, 30, 60, 120, 180, 300 s to measure APTT, PT, and FIB to determine the best blood collection time. Finally, the rats were divided into normal saline group and thrombin group (optimal thrombin dose and blood sampling time), and blood samples were collected to determine APTT, PT, FIB and whole blood viscosity.

　　Result: The plasma APTT and PT of rats in the 10 U/kg thrombin group were significantly shortened, FIB was significantly increased, and the mortality rate of rats was low. After 60 s of thrombin injection, the plasma APTT and PT shortened the most, and the FIB content rose to the highest. Compared with the saline group, the plasma PT and APTT of rats in the thrombin group were significantly shortened, and the FIB and whole blood viscosity increased significantly, and the difference was significant (P<0.05).

　　Conclusion: Injection of thrombin solution can replicate the rat blood hypercoagulability model. The best thrombin dose is 10 U/kg, the thrombin concentration is 2 U/mL, and the best blood sampling test time is 60 s.