小鼠肿瘤模型 z-bo 2020-12-24 315

　　Objective: To study the tumor targeting properties of the conjugated compound of farnesyl thiosalicylic acid and heptamethine cyanine near-infrared fluorescent dye and its application in in vivo imaging, and to determine the inhibitory effect of the compound on tumor growth. Methods: After human breast cancer cell MCF-7, glioma cell U251 and prostate cancer cell PC3 were cultured to the logarithmic growth phase, FTS and FTS-IR783 were added at different concentrations to observe the growth inhibition of the two compounds on tumor cells Effect: Add FTS-IR783 (20 μmol/L) to the three cultured tumor cells, observe the aggregation of fluorescent dyes in the tumor cells under a fluorescence microscope; transplant the three tumor cells (1×106 each) subcutaneously into nude mice Two weeks later, the tumor-bearing mice were injected with FTS-IR783 (10 nmol/L each) into the intraperitoneal cavity, and the correlation between the near-infrared fluorescence signal of the tumor site and the tumor volume was determined by in vivo imaging.

　　Results: Compared with FTS, FTS-IR783 can significantly inhibit the growth of MCF-7, U251 and PC3; three kinds of tumor cells can specifically recognize FTS-IR783, showing near-infrared fluorescence aggregation; subcutaneous tumor model after injection of FTS-IR783 , In vivo imaging showed that the correlation between the fluorescence intensity and bioluminescence intensity at the tumor site reached 0.987, 0.998 and 0.971, respectively. Conclusion FTS can specifically recognize tumor cells after being conjugated with the near-infrared fluorescent dye IR-783, which can be used for in vivo imaging of tumor models. At the same time, the compound has tumor targeting properties that can significantly inhibit the growth of tumor cells and is expected to become a new type Targeted drugs.