皮下移植瘤模型 z-bo 2020-12-24 375

　　Purpose: To explore the differences in subcutaneous transplantation tumors of lung cancer in different locations, and to provide basic evidence for lung cancer researchers. Methods: Mouse Lewis lung adenocarcinoma cells (ll2-luc-m38) stably expressing luciferase were injected into the right axillary subcutaneous, right inguinal subcutaneous, and foot pad subcutaneous of C57 BL/6 mice. The animal in vivo imaging system observes the tumor formation and metastasis of subcutaneous transplanted tumors in mice, observes the survival time and mortality of tumor-bearing mice in different parts, and draws tumor tissues, uses paraffin embedding, sectioning, and HE staining to make pathological diagnosis. After the mice were sacrificed, the lung tissue was fixed and the metastases were observed. The subcutaneous transplanted tumor was excised to observe the survival and metastasis of mice after surgery. Results: The tumors formed earlier in the axillary group and the groin, with a tumor formation rate of 100%, and the foot pads formed tumors later, with a tumor formation rate of 33%; the tumor volume increased rapidly in the inguinal group and the axillary group, among which the inguinal group The volume increased fastest; 70% of the mice in the armpit group had lung metastases on the 21st day after vaccination, and the number of metastases was large; 50% of the mice in the inguinal group had lung metastases, and the number of metastases was small; the foot pad group was small No lung metastases were observed in the mice. The mice in the footpad group had the highest mortality rate. Mice in the groin group and the armpit group can undergo subcutaneous tumor resection, and the postoperative survival rate is 100%. Conclusion: In the mouse Lewis lung cancer subcutaneous transplantation tumor model, the inguinal group and axillary tumors have a high rate, can tolerate the transplantation tumor resection, the risk of surgical death is low, it is easy to monitor, the operation is simple and the repeatability is high. Better transferability.