主动脉夹层模型 z-bo 2020-12-24 337

　　Objective: To establish a mouse aortic dissection model by using β-aminopropionitrile drinking water combined with angiotensin II subcutaneous pump. Methods: Forty male C57Bl/6J mice of 3 weeks old were randomly divided into two groups. All were given 0.1 g/(kg·bw) BAPN drinking water daily. On the 28th day after BAPN drinking, they were given a subcutaneous pump, BAPN drinking water + saline The submerged pump group continuously pumped normal saline, and the BAPN drinking water + Ang Ⅱ submerged pump group continuously pumped Ang Ⅱ at 1000 ng/(kg·bw) per minute. The blood pressure and heart rate of mice were detected by the tail cuff method of mouse non-invasive blood pressure meter. In the experiment, the dead mice were dissected immediately and the aorta was isolated. 72 hours after being buried in the pump, the mice that did not die were killed by an overdose of sodium pentobarbital, and the aorta was retained. The formation of false lumen in the aortic wall was observed under hematoxylin-eosin staining microscope. Results: The total incidence of dissection in the BAPN drinking water + Ang Ⅱ buried pump group was 95%, and the mortality rate was 24% (dissecting aneurysm rupture). The incidence of dissection was 5% in the BAPN drinking water + saline buried pump group, and death The rate was 0; the pathological section of the aorta under the microscope showed that the dissected aorta had false lumen.

　　 Conclusion: Successfully established a mouse model with a high incidence of aortic dissection.