DBA/1J小鼠胶原诱导关节炎模型 z-bo 2020-12-24 871

　　Objective: To establish a DBA/1J mouse collagen-induced arthritis model, and to identify the model from morphology, pathology and immunology.

　　Method: Mix bovine type Ⅱ collagen (CⅡ) with adjuvant and fully emulsify it, subcutaneously inject into the base of the tail of DBA/1J mice for the primary immunization, 21 days later, perform the same method for re-immunization. Observe the morphological indicators such as the weight change and the degree of joint swelling in the mice, perform pathological examination of the ankle joint, and use the CBA method to detect the level of serum cytokines.

　　Result: The body weight of the model mice began to decrease 35 days after the first vaccination and continued to be lower than that of the control group. On the 28th day, the model mice developed redness and swelling of the feet. The thickness of the feet swelled at 35 days was significantly higher than that of the control group (P<0.05), and the incidence rate was as high as 90% by 62 days. Pathological examination showed that the joint cavity of the model mice became narrow, the synovial tissue proliferated, inflammatory cells infiltrated, and the cartilage structure was destroyed to varying degrees. Compared with the control group, the inflammatory factors IL-6, IFN-γ, and MCP-1 in the serum of model mice increased significantly (P <0.05), while="" the="" anti-inflammatory="" factor="" il-10="" decreased="" slightly="" p="">0.05) .

　　 Conclusion: Subcutaneous injection of bovine type II collagen at the tail root of DBA/1J mice can successfully induce CIA model.