高动力型感染性休克犬模型 z-bo 2020-12-24 335

　　Purpose: To prepare a high dynamic septic shock dog model by intravenous injection of E. coli.

　　Method: 22 mongrel dogs were randomly divided into control group (n=11) and experimental group (n=11). After anesthetizing the dogs, a double-cavity central venous catheter was inserted through the right femoral vein. The control group used a micro pump to pump normal saline into the dog’s femoral vein. The experimental group was pumped with Escherichia coli in the same way, and a PICCO catheter was inserted through the right femoral artery. PICCO was used to monitor systemic hemodynamics at various time points between 0 and 48 h. Results: The heart rate (HR), cardiac output (CO), and stroke volume (SV) of the experimental group were significantly increased at 12 h (P<0.05), and the systolic blood pressure (SBP) and peripheral vascular resistance (SVR) were at 12 h. h was significantly reduced (P <0.05), while="" other="" hemodynamic="" indicators="" such="" as="" central="" venous="" pressure="" pulmonary="" artery="" wedge="" oxygen="" delivery="" consumption="" uptake="" rate="" were="" different="" none="" was="" significant="" p="">0.05). A significant decrease in urine output can be observed in the experimental group from 12 h; peak blood flow velocity (PSV) and resistance index (RI) during arterial systole are significantly different from those in the control group (P<0.05).

　　 Conclusion: The use of a micro-pump to pump live E. coli into the canine femoral vein can successfully prepare a highly dynamic septic shock dog model, which can provide an experimental basis for the study of septic shock.