大气细颗粒物致炎动物模型 z-bo 2020-12-25 329

　　Objective: To explore the pathogenic mechanism of air pollution on animals, and to use non-invasive tracheal instillation of PM2.5 particle suspension in BALB/c mice to construct an animal model of air pollution caused by inflammation? Methods: 150 SPF BALB/c mice were randomly divided into blank control group, saline group, PM2.5 low group (2.5 mg/kg), PM2.5 moderate group (5 mg/kg) and PM2. 5 Altitude group (10 mg/kg), a total of 5 groups, in each dose group, on day 3, day 7, day 21, day 35, day 49, tissue samples were taken 24 hours after tracheal infusion operation was completed , Using ELISA? lung tissue pathological HE staining method to verify the feasibility of the non-invasive tracheal instillation method and the success of the inflammatory model? Results: The success rate of this modeling method is as high as 96%. Using the tracheal instillation method, the lung tissue inflammation score of modeling mice is positively correlated with the prolongation of airway instillation time and the dose. After PM2.5 exposure, there is a large amount in the lungs. Lymphocyte aggregation and macrophage infiltration of phagocytic granules, widening of alveolar space? Each exposure group was compared with the normal saline control group? Blank group, the inflammatory factor IL-6 in the alveolar lavage fluid and TNF-α in the lung tissue homogenate With higher levels, the high-dose group has the most significant difference?

　　 Conclusion: In this experiment, the tracheal instillation method was used to establish a mouse model of inflammation, and proved that this method is simple, reliable, and can be widely used for repeated instillation of the mouse respiratory system. It is beneficial to further research on air pollution and other inflammatory mechanisms?