食蟹猴肌肉萎缩模型 z-bo 2020-12-25 517

　　Purpose: To use the method of restricting lower limb activities to establish an animal model of cynomolgus monkey muscle atrophy and establish a method for model evaluation? Method: Fix the right lower extremity (ankle to knee joint) of cynomolgus monkey with a polymer fiber bandage for 15 weeks. During this period, measure the body weight, the circumference of the bilateral calf and the volume of the lower extremity every two weeks. MRI of gastrocnemius muscle, muscle biopsy at 15 weeks?

　　Results: After 15 weeks of modeling, there was no significant change in animal body weight. The volume of right calf and leg circumference, right thigh and leg circumference, and right lower extremity decreased significantly compared with before modeling (P<0.05); 15 weeks after modeling, serum creatinine and blood sugar? Alkaline phosphatase was significantly decreased (P <0.05), and serum cholesterol levels were significantly increased (P <0.05); MRI showed atrophy of the right gastrocnemius muscle, and the volume of the right gastrocnemius muscle decreased compared with before modeling; muscle tissue biopsy showed the right The gastrocnemius muscle is atrophy and muscle fibers become thinner; the cross-sectional area of gastrocnemius muscle fibers decreases Conclusion: The muscular atrophy model is made by external fixation to restrict the movement of the lower extremities of the cynomolgus monkey. The modeling cycle is appropriate and the operability is strong.

　　Comprehensive use of leg circumference, lower extremity drainage volume, biochemical indicators, MRI imaging and muscle biopsy combined evaluation method, Can it be simple? Objectively evaluate the cynomolgus monkey muscle atrophy model?