全子宫切除模型 z-bo 2020-12-26 555

　　Objective To establish an experimental animal model of BALB/c mouse total hysterectomy, and to explore and analyze the characteristics and formation mechanism of the model.

　　Methods were divided into blank control group, sham operation group and model group. The blank control group received no treatment and was kept routinely; the model group underwent total hysterectomy via the abdomen; the sham operation group only had the skin cut and the uterus was exposed.

　　Routine rearing after operation , Dynamic observation of the weight changes of mice in each group, and detection of peripheral blood estradiol content and ovarian histological changes at the end of 4 weeks. Results After the operation, all experimental mice in each group survived, with good general symptoms, no obvious inflammation of the incision, and complete healing; there was no significant difference in the preoperative weight of the experimental mice in each group. The second week after the operation, the third week and the fourth week, The body weight of mice in the model group was higher than the control group and the sham operation group (P<0.05); the peripheral blood estradiol content of the model group was significantly lower than the control group and the sham operation group (P<0.01); the blank control group and the sham operation group The histological examination of the ovaries of the mice in the operation group showed no abnormalities. The model group showed abnormal histological changes such as irregular follicles, loose granular cells, and apoptosis.

　　Conclusion This experimental animal model can well simulate the clinical manifestations of postoperative ovarian function decline and is an ideal animal model for studying complications after hysterectomy and screening drugs.