动物造模,十二指肠溃疡 z-bo 2022-02-02 459

　　[Modeling mechanism] Cysteamine, also known as β-mercaptoethylamine, can specifically consume human somatostatin. It is also called a somatostatin depletion agent. D-cell damage to the duodenal mucosa may be one of the targets of the selective action of cysteamine. D-cell damage will increase local gastric acid secretion and may cause duodenal ulcer. [Modeling method] Sprague Dawley rats with an average weight of 200 g and 10% cysteamine hydrochloride in water were given by gavage or subcutaneous injection. The model of acute duodenal ulcer is oral administration of 10% cysteamine 28mg/100g x 3 times, 40mg/100g x 2 times or 75mg/100g x 1 time, 10% cysteamine 20mg/100g subcutaneous injection. ×2 times. Each interval is 3 hours. The model of chronic duodenal ulcer is oral administration of 10% cysteamine 28mg/100g x 3 times or 40mg/100g x 2 times (interval 3-4 hours each time), and then oral administration of 0.2% or 0.05% or 0.01%. Aqueous cysteamine hydrochloric acid. As a result, on the third day of the acute model, more than half of deep cysteamine ulcers were observed orally or subcutaneously or from the pylorus to the pylorus, 2mm to 4mm from the pylorus, and there were inflammatory cell infiltration and necrotic tissue fragments at the ulcer site. I know. , And even ulcerative perforations (perforation of the anterior wall of the pancreas and perforation of the posterior wall of the liver). In the second to third weeks of chronic modeling, the mucosal surface is rough, there are many fibrous connective tissues around the ulcer, and there is epithelial hyperplasia at the edge of the ulcer.

　　[Characteristics of the model] Ulcers are very limited to the anterior and posterior walls of the duodenum. Subcutaneous injection of cysteamine has the same effect as oral cysteamine, indicating that cysteamine has a specific effect. I'm. Locally located in the duodenum. In the chronic model, rats were given 0.2%, 0.05% or 0.01% cysteamine hydrochloride aqueous solution to maintain a low gastric pH and promote the development of duodenal ulcers. This is also the clinically low pH in the stomach of patients with duodenal ulcer.

　　[Model evaluation and application] Patients with hypergastrinemia and adrenal cortex disease have limited ulcer development and multiple perforations, as well as the pathomorphological characteristics of duodenal ulcers are very similar. Cysteamine is widely used to simulate gastric and duodenal ulcers, and is of great significance in analyzing the neuroendocrine mechanism of ulcers.