胰腺癌原位移植模型 z-bo 2020-12-28 349

　　Objective: To establish an orthotopic transplantation model of pancreatic cancer based on clinical tumor specimens, and to carry out model evaluation research.

　　Methods: Transplanted clinically fresh surgical specimens of pancreatic cancer to the pancreas of nude mice, established orthotopic xenotransplantation model (PDOX), assessed the tumor growth by in vivo imaging technology and PET/CT; analyzed the source of transplanted tumors by STR technology To determine the pathological characteristics of transplanted tumors through histomorphological observation and immunohistochemistry, and to detect the expression level of CA19-9 in the peripheral blood of the PDOX model.

　　Results: Six weeks after the model was established, the near-infrared fluorescence signal was observed to be significantly enriched in the tumor site through in vivo imaging. The tumor location and size can be preliminarily judged by the fluorescence intensity; the abdomen 18 can be clearly observed using small animal PET/CT The molecular probe enrichment area is consistent with the expected tumor location and size; after euthanasia, the separated organs and tumor tissues are dissected and the tumor growth status is further confirmed by near-infrared fluorescence imaging; STR testing confirms that the transplanted tumor is human The sex ratio was 99%. Histomorphology and immunohistochemical analysis showed that the transplanted tumor grew on the pancreas of nude mice. The CA19-9 detection in serum further confirmed the occurrence of pancreatic cancer.

　　Conclusion: A human pancreatic cancer orthotopic xenotransplantation model was successfully established. The model was comprehensively evaluated through small animal in vivo imaging, PET/CT and other technologies, which provided a good animal model for the pathogenesis and treatment of pancreatic cancer.