胃炎癌转化模型 z-bo 2020-12-28 321

　　OBJECTIVE: To construct a rat gastritis cancer transformation model and explore new early serological diagnostic indicators for gastric cancer.

　　Method: Using N-methyl-N'-nitro-N'-nitrosoguanidine drinking water (MNNG) and high-salt feed feeding method: induction of rat gastritis cancer transformation model. MRI and histopathological observations Deteriorating process of rat stomach. qRT-PCR detects the expression levels of miR-17-5p and miR-374-5p in serum of rats at different time points (0, 1 24, 36, and 48 weeks).

　　Results: MNNG drinking water and high-salt feed successfully induced gastritis cancer transformation in rats, and experienced atrophic gastritis (24th week of modeling) and atypical hyperplasia (48th week of modeling). The serum miR-17-5p and The expression level of miR-374-5p is positively correlated with the degree of gastric deterioration in rats. Compared with the normal control group, the expression level of miR-17-5p increased by about 8 times at 36 to 48 weeks (t=4.1 P< 0.001) and 10 times (t=5.3 P<0.001); at the 48th week, the expression level of miR-374-5p increased by about 6 times (t=3.6 0.001<P<0.002).

　　Conclusion: The rat gastritis cancer transformation model constructed by MNNG drinking water and high-salt feed feeding provides a good animal model for studying the pathogenesis of gastric cancer. Serum miR-17-5p and miR-374-5p are potential early noninvasive diagnostic indicators for gastric cancer.