进展期肝脏肿瘤动物模型 z-bo 2020-12-28 331

　　Objective: To establish a new type of advanced liver tumor (hepatocellular carcinoma, colorectal cancer liver metastasis, breast cancer liver metastasis, and non-small cell lung cancer liver metastasis) animal models by injecting tumor cells into the portal vein.

　　Methods: Human hepatocellular carcinoma MHCC97-H, human colorectal cancer SW480, human non-small cell lung cancer A549, and human triple-negative breast cancer MDA-MB-231 cell lines were cultured, and these malignant tumor cells were exposed to BALB/c. The mouse hepatic portal vein is injected into the liver, and the PET/CT test is performed after 3~4 weeks of growth: about 200μCi nuclide probe 18F-FDG is injected into the tail vein, and the BALB/c nude mice are subjected to PET/CT test 30~40min. On this basis, collect animals to obtain livers and take pictures to determine the tumor lesions on the liver of BALB/c nude mice.

　　Results: Cells such as MHCC97-H, SW480, A549 and MDA-MB-231 can form multiple and diffuse tumor foci in the liver of BALB/c nude mice. Tumor cells can grow in situ in the liver of nude mice using PET/ CT detection. For lesions formed by SW480, MDA-MB-231 and A549 cells, the PET/CT detection signal is weaker than that of MHCC97-H cells.

　　Conclusion: A new type of advanced liver tumor animal model was successfully obtained, laying a solid foundation for the screening of drugs related to advanced liver tumors.