大鼠内脏高敏感模型 z-bo 2020-12-29 272

　　Objective: To establish a rat visceral hypersensitivity animal model by foot electric shock, and evaluate its effectiveness and advantages.

　　Methods: Female SD rats were randomly divided into normal control (NC) group, plantar electric shock (FSS) group and water avoidance stress (WAS) group. The NC group was placed in a simulated stress box every day for 1 hour without stimulation; FSS The group was placed in an electric shock box for 1 hour a day and given electric shock stimulation. The stimulation voltage was 40V, and the stimulation frequency was 20 times/min, 1s each time for 5 minutes; the WAS group was given 1 hour water avoidance stress every day; the modeling period was 10 days. Observation Record the general condition and defecation of the rat, use the abdominal wall retraction reflex (AWR) to detect the visceral sensitivity, use the ELISA method to detect the serum CRF, ACTH, CORT, 5-HT concentration and colon tissue 5-HT concentration.

　　Results: (1) The visceral sensitivity of the rats in the FSS group was significantly higher than that of the WAS and NC groups (P<0.05), and there was no obvious pathological damage to the colonic mucosa. (2) The serum CRF, ACTH, CORT, and 5 of the rats in the FSS group -HT concentration and colon tissue 5-HT concentration were significantly higher than those of WAS group and NC group (P<0.05). (3) The defecation volume of rats in FSS group was significantly higher than that of WAS group and NC group (P<0.05). The water content of feces was significantly higher than that of the NC group (P<0.05), and the values of stool volume and fecal water content of rats were stable.

　　Conclusion: The plantar electric shock modeling method can establish a rat visceral hypersensitivity model and is better than the water avoidance stress method.