溃疡性结肠炎模型 z-bo 2020-12-29 307

　　OBJECTIVE: To explore the molecular connotation of the theory of traditional Chinese medicine through papaverine regulation of respiratory diseases and inflammatory bowel disease. Analyze the changes of differentially expressed genes in two animal models of diseases after treatment to reveal the molecular mechanism of lung-intestinal linkage

　　Methods: The ovalbuminOVA nebulization method was used to establish an allergic asthma model and compared with the ulcerative colitis model established by the compound immune method. Normal rats were used as a control. After papaverine administration, reverse transcription polymerase was used Chain reaction technology (RTPCR) to detect the mRNA expression levels of common differentially expressed genes in the lung tissue and intestine tissue of the two groups of model rats

　　Results: Papaverine can significantly improve the pathological changes of rat lung tissue and colon tissue. Through KEGG annotation analysis and sequencing results, three key differentially expressed genes were finally obtained: C￣C motif chemokine 11 carbonic anhydrase 1 nuclear hormone receptor sub Family D group member 1 RT￣PCR results are basically the same as the transcriptome sequencing results up and down, and papaverine significantly reduces the expression of Ccl11 mRNA in the lung and colon tissues of the two groups of model rats (P<0 05) and increases Ca1 and Nr1d1 mRNA expression level (P <0 05 P <0 05)

　　Conclusion: The lungs and intestine tissues of rats with respiratory diseases and inflammatory bowel disease have the same differentially expressed genes. Papaver somniferum can significantly regulate the level of differentially expressed genes between the lung and the large intestine. This may be one of the molecular mechanisms of Papaver somniferum returning to the lung and the large intestine meridian.