大鼠COPD-OSAHS重叠综合征模型 z-bo 2020-12-29 299

　　Objective: To construct a rat model of COPD-OSAHS overlap syndrome and provide feasible means and experimental platform for basic research on OS.

　　Methods: Fourteen SPF female 6-week-old SD rats were randomly divided into experimental group and control group. The experimental group (8 rats) were exposed to smoking and intermittent hypoxia, and the control group (6 rats) were treated with fake smoking and Normal oxygen exposure. Calculate the survival rate of rats in the experimental group after 8 weeks, and compare the general conditions of the two groups of rats, blood gas analysis, right ventricular hypertrophy index, average alveolar number, average alveolar interval, lung tissue pathological changes.

　　Results: The oxygen concentration-time variation curve of the intermittent hypoxia box showed that the oxygen concentration periodically decreased and returned to normal, which successfully simulated the intermittent hypoxia environment; the experimental group eventually survived 6 with a survival rate of 75%, and the control group 6 All the rats survived. Compared with the control group, the 6 surviving SD rats in the experimental group were generally in poor condition, with acidosis, hypoxemia, carbon dioxide retention, right ventricular hypertrophy, and mean alveolar intercept increased significantly, and mean alveoli The number was significantly reduced (P<0.05), and the difference was statistically significant; pathological observation of lung tissue showed inflammation infiltration of lung interstitium, lymphocytic proliferation of bronchial wall, smooth muscle hyperplasia of bronchiolar wall, partial breakage of smooth muscle of tube wall, goblet cell hyperplasia and Emphysema.

　　Conclusion: The rat OS model was successfully established by smoking combined with intermittent hypoxic exposure. More meaningful and objective model evaluation indicators need to be discovered by research.