支气管哮喘模型 z-bo 2020-12-29 308

　　OBJECTIVE: To investigate the effects of different doses of ovalbumin (OVA) on serum IgE, IL-4, IFNγ content, bronchoalveolar lavage fluid (BALF) eosinophils, and serum IgE, IL-4, IFNγ levels in BALB/c mouse bronchial asthma model. EOS) quantity and the influence of lung histopathology, preliminary determination of a more appropriate OVA model dosage.

　　Methods: BALB/c mice were randomly divided into normal group, model 1 group (OVA20μg), model 2 group (OVA50μg), model 3 group (OVA100μg), dexamethasone acetate group (OVA100μg). Except for the normal group, the others Group mice were sensitized by intraperitoneal injection of corresponding doses of OVA and challenged with 1% OVA atomization to establish a bronchial asthma model. ELISA method was used to detect serum IgE, IL-4, IFN-γ levels, and Wright staining to calculate the number of EOS in BALF HE staining to observe the pathological changes of lung tissue.

　　Results: Compared with the normal group, serum IgE, IL-4, IFN-γ and EOS in BALF of mice in each group increased significantly (P<0.05), and inflammatory cell infiltration and bronchial epithelial cell degeneration appeared in lung tissues. Pathological changes such as proliferation of bronchial smooth muscle cells are more obvious in the content of IgE, IL-4, IFN-γ and lung tissue pathological changes in the OVA50μg group.

　　Conclusion: Three doses of OVA can successfully induce asthma in BALB/c mice. In general, the asthma model induced by 50 μg OVA is more obvious. It is recommended as an appropriate dose for BALB/c mouse asthma model.