自身免疫性重症肌无力小鼠模型 z-bo 2020-12-29 313

　　Objective: To establish an experimental autoimmune myasthenia gravis animal model that meets the international preclinical experimental standards.

　　Method: After referring to the method reported in the literature and improved, the pure acetylcholine receptor (AchR) protein was extracted from the electric ray organ, and the protein was qualitatively identified by SDS gel electrophoresis and the protein was quantified by the BCA method; the purified protein was used for the initiative Immunize C57BL/6 mice for a total of 3 times (respectively on day 1, 30, and 60), and perform EAMG mouse clinical score, body weight, serum AchR antibody content, neostigmine test, and electromyogram And other comprehensive evaluation.

　　Results: Comparing the EAMG model group with the adjuvant group, the disease started from the third week, and the average clinical score increased significantly (P<0.01); the weight of the diseased mice was significantly reduced (P<0.01); the neostigmine test was positive; serum AchR The antibody content increased significantly (P<0.01); the electromyogram repeated electrical stimulation test was positive.

　　Conclusion: Extracted from the electrical organs of the black-spotted double-fin electric rays and purified AchR protein successfully induced the C57BL/6EAMG mouse model, which created good conditions for further research on myasthenia gravis.