大鼠深静脉血栓模型 z-bo 2020-12-29 410

　　Objective: To observe the expression and significance of thrombomodulin (TM) during the natural evolution of deep vein thrombosis and regression in rats.

　　Methods: SD rats were randomly divided into a model group (n=60) and a control group (n=6). The model group adopted the "stenosis method" to establish the DVT model, and the control group adopted the "sham operation method". 1h, 4h, 6h, 12h, 24h, 3d, 7d, 14d, 21d, 28d at 10 time points. Rats were tested for thrombus weight/length ratio, soluble TM concentration in plasma, TMmRNA in thrombus tissue and inferior vena cava endothelium expression. Results: 1) Thrombus weight/length ratio was low in the early stage of modeling (1, 4, 6 and 12h), and there was no significant difference between the four groups (P>0.05); it was stable at 24h, 3d, and 7d after modeling A higher level (P>0.05); 14 days after modeling, it began to decrease, but it was not significantly different from the results of 21 and 28 days (P>0.05). (2) ELISA results showed that the soluble TM in plasma was higher than the control group High, the difference is significant (P<0.01), and the overall increase gradually over time. (3) The real-time fluorescent quantitative PCR results show that the expression of thrombus TMmRNA gradually increases over time. The expression of vascular endothelial TMmRNA is 1 ､4､6､12h was higher than the control group (P<0.01); at 24h, 3d, 7d was lower than the control group (P<0.01); 28d="" compared="" with="" the="" control="" difference="" was="" not="" significant="" p="">0.05). (4) Thrombus weight/length ratio is negatively correlated with endothelial TMmRNA (r=-0.92, P<0.01); while thrombus TMmRNA and plasma soluble TM are positively correlated (r= 0.96, P<0.01).

　　Conclusion: The expression changes of endothelial TMmRNA can reflect the progression of DVT and have a good prognostic effect.