结肠癌肝转移裸鼠模型 z-bo 2020-12-29 329

　　Objective: To construct a colon cancer liver metastasis model with high metastasis rate, simple operation, and reliable results for experimental research on the prevention and treatment of colon cancer metastasis.

　　Method: 15 Balb/c nude mice were divided into 3 groups (group A, group B, group C), and 5 Balb/c mice were divided into group D. The HCT116 and CT26 cells with a cell concentration of 2.5×107/mL Suspension 0.2mL was used to construct colon cancer liver metastasis model by spleen planting and spleen cutting method, and the success rate of four groups of animal models and the size and number of liver metastases and intra-abdominal metastasis were compared.

　　Results: The success rate of nude mice modeling in group A was 100% (5/5). The liver and spleen were tumors. The number of liver metastases was less and scattered, and they were mostly distributed in the right lobe of the liver. The average survival time was (26.6±3.4). )d; The success rate of nude mice modeling in group B was 40% (2/5), the metastatic tumors were scattered on the liver surface, the volume was larger than that in group A, and the average survival time was (36.8±4.2) d; nude mice in group C were successfully modeled The rate was 100% (5/5). Both the liver and spleen became tumors. There were a large number of liver metastases, and multiple metastases fused into clusters, occupying the entire right lobe of the liver, and the average survival time was (20.2±2.6) days; group D No metastasis was found in the liver. Part of the three groups of nude mice showed abdominal metastasis (2 in group A, 3 in group C), and no heart, lung, brain, or kidney metastasis appeared in the three groups of nude mice. Histocytology of liver metastases in the three groups Meet the characteristics of adenocarcinoma.

　　Conclusion: The spleen-preserving method can achieve a higher success rate of modeling, and can effectively simulate the way and process of human colon cancer cells metastasizing to the liver through blood.