动物造模 z-bo 2022-02-01 461

　　[Modeling mechanism]: The proliferation, metastasis and recurrence of gastric cancer depend on angiogenic factors. There are many factors affecting the transplantation of human gastric cancer into nude mice, including human gastric cancer tissue and cells transplanted into nude mice. And is widely recognized. Vascular endothelial growth factor, nitric oxide, cyclooxygenase 2, etc.

　　[Modeling method]

　　1. Anesthetize nude mice weighing 18 to 20 g with thiopental sodium for 3 to 5 weeks, and after opening the stomach, inoculate human gastric cancer cell SGC-7901 suspension (cancer cells inoculate 0.2ml. The number is about 1,000,000) .

　　2. Cut the surgically removed gastric cancer tumor tissue into 1 cubic millimeter and place it under the skin of the back of the nude mouse. After the tumor is formed, the subcutaneous channel is repeated 3 to 4 times, so that the tumor grows stably under the skin and the diameter increases to 1-2 cm. The tumor-bearing nude mice were sacrificed. Aseptic operation. The nude mice were dissected, the subcutaneous tumor was completely removed, and the subcutaneous tumor was cut into 1-2 cubic millimeters. After anesthetizing the nude mice to be transplanted, cut all layers of the abdominal wall to expose the stomach wall, carefully cut out the serosal layer with large curvature of the stomach, remove the serous membrane and implant it in the tumor, use the existing tumor tissue block to treat with glue Paste and repair. The stomach returns to the abdominal cavity and the abdomen is closed.

　　[Characteristics of the model]: 3 days after the inoculation, the inoculation site was pale and hard, and 1 week after the inoculation, the nodules in the mucosal inoculation site were invaded. Two weeks after the inoculation, the stomach was raised and became uneven, and gastric mucosal folds were found. Partly disappeared, local lymph nodes were enlarged, and a small amount of ascites was found in the abdominal cavity. Three weeks after inoculation, the stomach mass hardened and solidified in the surrounding colon and spleen, indicating that cancer cells had invaded the mucosa and muscle layers. Serosal growth and involvement. The establishment of an orthotopic transplantation model of human gastric cancer in nude mice with immunodeficiency animals has strict requirements on the care conditions, environmental control, diet and water supply of laboratory animals. In addition, the treatment of gastric cancer tissue in the process of tumor tissue mass transplantation is another important factor affecting the success of the model. [Model evaluation and application] The established nude mouse gastric vaccination model of human gastric cancer reproduced the pathological process of clinical gastric cancer with local infiltrating lymph node metastasis and intraperitoneal spread, providing a new method for gastric cancer research. Made a closer animal model. We have established an orthotopic transplantation model of human gastric cancer to reproduce the mechanism of gastric cancer, effectively screen anti-tumor drugs, study the role of various factors in the development of gastric cancer, and study the growth and metastasis of transplanted tumors. You can learn to influence.