人柯萨奇B2病毒感染恒河猴模型 z-bo 2020-12-30 296

　　Purpose: Through the preliminary analysis of the infection characteristics of human coxsackie B2 virus infected rhesus monkeys through the oral cavity, to provide experimental methods and technical support for the establishment of the human coxsackie B2 virus rhesus monkey infection model, and for subsequent development. Lay the foundation for research on infection mechanism and pathophysiology.

　　Methods: Coxsackie B2 virus was used to infect infants of rhesus monkeys aged 3 to 4 months by oral infection, and the clinical symptoms and body temperature of rhesus monkeys were observed and recorded; blood was collected for physiological and biochemical indicators; blood samples and herpes were tested for virus; herpes tissues Perform pathological testing.

　　Results: Two to five days after infection, rhesus monkeys developed hand, foot, and mouth herpes; animals showed depression, reduced activity and diet; body temperature showed a missed heat curve; Coxsackie could be detected in herpes fluid and blood samples B2 virus; routine blood tests showed a decrease in white blood cells, an increase in monocytes, a decrease in the number of lymphocytes, an increase in the number of granulocytes, an increase in eosinophils, and a decrease in basophils. Blood biochemical tests found that liver function, kidney function, and myocardial enzymes all increased to varying degrees; herpes histopathology showed that rhesus monkey herpes showed thickened squamous epithelium, hyperkeratosis on the surface, local necrosis with inflammatory cell infiltration, and abscesses. form.

　　Conclusion: A series of more characteristic hand-foot-mouth herpes, clinical symptoms, physiology and biochemistry, viremia and other conditions appeared after human Coxsackie B2 virus infection in infant monkeys, indicating that the oral infection method of rhesus monkey infants can successfully construct Coxsackie The animal model of B2 virus infection, the established related detection methods and technologies are feasible, and it has laid the experimental foundation for the subsequent establishment of the Rhesus monkey Coxsackie B2 virus infection model, pathogenesis research, drug and vaccine evaluation.