动物模型 z-bo 2020-07-31 525

　　With the continuous deepening of oncogene research, the application of transgenic technology in oncology has become more and more extensive. Transgenic gastric cancer model is a model in which related genes regulating gastric cancer are directly transfected into animal embryos to form tumors. [Modeling method] A method of forming tumors by transplanting oncogenes or proto-oncogenes (such as cells and viruses) into rat embryonic tissues. The morphological characteristics of tumors formed in this way are similar to natural human tumors and have very high specificity. The tumor only occurs in certain tissues, such as adenovirus 12 Ela/Elb transgenic rats, and forms a human-like natural gastric cancer at the junction of squamous epithelium and columnar epithelium. Using the 424 bp long CEA promoter, gastric adenocarcinoma mice produced with the transgenic SV40 T antigen had tumors in the pylorus, and the tumor incidence was 100%. [Model Evaluation and Application] The development and development of gastric cancer is a multi-factor, multi-step process.

　　The traditional modeling results show that the combination of MNNG and other related gastric cancer factors can significantly increase the induction rate of cancer and shorten the time to form gastric cancer. However, with the advent of transgenic gastric cancer models, modern molecular biotechnology can be used to transplant oncogenes and proto-oncogenes derived from cells and viruses into rat embryonic tissues to form tumors. To some extent, gastric cancer, which is similar to the spontaneous development of humans, forms at the junction of squamous epithelium and columnar epithelium. This is an ideal gastric cancer model, because genes that regulate gastric cancer are directly transfected into animal embryos to form tumors with morphological characteristics similar to human gastric cancer, and used for future gastric cancer research. This will be the main model.