小鼠肝癌模型 z-bo 2020-12-30 342

　　Objective: To explore the mechanism of liver cancer by establishing a chemically induced liver cancer model in C57BL/6 mice to simulate the occurrence and development of liver cancer. Methods: 110 C57BL/6 mice were selected and randomly divided into a control group (30) and a model group (80). The mice in the control group were not treated. The model group was established by intragastric administration of CCl4 combined with intraperitoneal injection of DEN Liver cancer model; continuously measure the body weight of mice, dissected mice at different time nodes to observe liver morphology, measure the level of liver function-related enzymes in venous blood serum, and observe the pathological changes of liver tissue.

　　Result: Compared with the control group, the mice in the model group gradually decreased their body weight, had abnormal liver morphology, and elevated serum levels of liver function-related enzymes ALT and AST, and had obvious histopathological changes in liver cancer.

　　Conclusion: Diethylnitrosamine (DEN) combined with carbon tetrachloride (CCl4) can induce liver cancer in C57BL/6 mice, providing a reliable animal model for studying the molecular mechanism of liver cancer.