大鼠重度创伤失血性休克模型 z-bo 2021-01-04 285

　　Objective To establish a modified rat model of severe trauma and hemorrhagic shock, and to explore the protective effect of anesthesia on the establishment of STHS rat model.

　　Methods Forty adult healthy male SD rats were selected and randomly divided into four groups: the normal group was anesthetized with bilateral femoral artery and left femoral vein catheterization, and the rats were awake or maintained anesthesia and divided into awake control group (CC, n = 10) The anesthesia control group (AC, n = 10) and the trauma shock group used open abdominal wounds combined with anesthesia or timed and quantitative controlled blood loss under 50% awake state to create severe traumatic hemorrhagic shock rat models, respectively, awake traumatic blood loss Sexual shock group (CTHS, n = 10), anesthesia traumatic hemorrhagic shock group (ATHS, n = 10). Observe the general physiological conditions of the rats in each group during the experiment, and continuously monitor the hemodynamic indicators of the experimental rats: mean arterial pressure (MAP), heart rate (HR), left ventricular pressure maximum rise/fall rate (± dp / dt) max), arterial blood gas indicators: pH, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hemoglobin concentration (ctHb), lactic acid (Lac), blood glucose (Glu), bicarbonate concentration (cHCO3-), Alkaline excess (BE); when reaching the end of the experiment, the damage of the rat's heart, liver, lung, and kidney organs was observed under the HE staining microscope.

　　Results There were significant differences in the changes of HR and ±dp / dt max in CTHS and ATHS groups over time (P<0.01). HR and ±dp / dt max in the ATHS group showed a decreasing trend, while the CTHS group decreased first (T1) Re-increasing or stable (T2, T3, T4, T5, T6) state; MAP showed a gradual upward trend after shock in the ATHS group, and eventually returned to a normal level of 25%-40%, while the CTHS group declined rapidly after a compensatory increase . PH, PaCO2, ctHb, cHCO3 -, BE in CTHS and ATHS groups showed a gradual downward trend. Except for PH and ctHb, the decline in CTHS group was significantly greater than that in ATHS group (P<0.01); Glu, Lac, PaO2 showed a gradual upward trend , And the increase in CTHS group was significantly higher than that in ATHS group (P<0.01). Compared with the CTHS group, the ATHS group had significantly lower liver, heart, lung, and kidney damage.

　　Conclusion Anesthesia has a protective effect on the establishment of severe traumatic hemorrhagic shock in rats. It can improve hemodynamics, reduce respiration and metabolite accumulation, and reduce the degree of damage to various organs.