非酒精性脂肪肝病模型 z-bo 2021-01-04 291

　　Objective To establish a non-alcoholic fatty liver disease model in a new type of hyperlipidemia susceptible rats, observe the changes of blood biochemical indexes and pathological characteristics, and explore its application and scientific research value, so as to provide a new choice for NAFLD disease animal models.

　　Methods Twenty WSHc rats aged 7-8 weeks were randomly divided into 2 groups and fed with normal diet and high-fat diet; another 20 Wistar rats of the same age were taken and treated in the same way as strain controls . After 12 weeks of continuous feeding, serum TC, TG, ALT and AST levels were detected, and elastic ultrasound imaging was performed. After the animals were sacrificed, the livers were taken for HE, Oil Red O, Masson and immunofluorescence staining. The morphology, lipid deposition, inflammation and fibrosis of the liver of each group were observed. The liver of WSHc rats after high-fat feeding was analyzed. Characteristics of lesions.

　　Results Compared with Wistar rats fed high-fat diet, WSHc rats were induced by high-fat, serum total cholesterol was significantly increased and was similar to that of clinical hyperlipidemia patients; liver lipids were abnormally deposited and formed bullous lipid droplets There are inflammatory cells infiltrate around lipid droplets to form inflammatory foci, and there are more macrophages; scattered fibrotic lesions can be seen. However, these pathological changes were not found in the liver tissue of Wistar rats induced by high fat.

　　Conclusion Compared with Wistar rats, blood lipids and liver enzymes in WSHc rats are close to clinical levels after high-fat induction. There are more serious lipid deposition and inflammatory reactions in liver tissues, and sporadic liver fibrosis, so it can be used as a first A new and ideal NAFLD animal model.