SD大鼠2型糖尿病模 z-bo 2021-01-04 304

　　Objective To investigate the preparation method of high-fat diet combined with streptozotocin-induced diabetes in rats, and provide a stable animal model of diabetes for clinical research.

　　Methods Thirty male SD rats were randomly divided into 2 groups: normal control group (NG, n = 10) fed with ordinary diet; type 2 diabetes group (DM, n = 20) fed with high fat diet (HFD) for 12 weeks +STZ 25 mg/kg injected through the tail vein. During the experiment, the body weight of the rats was measured weekly, and the changes in the oral glucose tolerance test (OGTT), insulin tolerance test (ITT) and the area under the blood glucose curve were observed one week before STZ injection. After STZ injection, observe the general conditions of the rats (food intake, water consumption, hair gloss, bedding moisture and mental status), and randomly detect blood glucose and fruit amine in rats.

　　Results Compared with the NG group, the body weight of the DM group increased significantly, and the difference was significant after 12 weeks of feeding (P<0.05). The blood glucose of OGTT experiment 60, 90, 120 min was significantly higher than that of the NG group (P<0.05), and the area under the curve was significantly increased (P<0.05); the blood glucose of the DM group in the IPITT test was significantly higher than that of the NG group (P<0 05), the area under the curve increases significantly (P<0.05). After STZ intervention, there were significant differences in food intake, drinking water and body weight between the two groups of rats (P <0.05). The random blood glucose and fructosamine in the DM group were significantly higher than those in the NG group (P <0.05).

　　Conclusion High-fat feeding rats caused insulin resistance and impaired glucose tolerance after 12 weeks. Combined with low-dose STZ-induced type 2 diabetes rat model with stable blood sugar, it is an ideal animal model for studying type 2 diabetes.