小鼠慢传输型便秘模 z-bo 2021-01-04 502

　　Objective To compare loperamide hydrochloride-induced slow transit constipation mouse models, compare the differences between the models induced by different doses and different administration times, and explore the establishment of stable loperamide hydrochloride-induced slow transit constipation mouse models. Experimental parameters.

　　Methods Healthy male C57BL/6 mice were used to prepare the loperamide hydrochloride suspension of 0.025, 0.25 and 2.5 mg/mL by intragastric administration. The administration time was 9 am and 3 pm every day , Give it twice. The effect of modeling was evaluated from the detection of intestinal propulsion rate and the detection of stool volume, and the effects of different doses and different administration times on the slow transit constipation mouse model were observed.

　　Results 1) Comparing the effects of different doses of loperamide hydrochloride in modeling, in the range of 0.5 mg/kg ~ 50 mg/kg, by comparing the intestinal propulsion rate between groups, the results show that 50 mg/kg Compared with the blank group, the intestinal propulsion rate and defecation volume of the mice were significantly reduced in the kg group, and the difference was statistically significant (P<0.05); (2) Compare the four differences of 3, 7, 10 and 14 days The number of days for modeling, on the 7th day of modeling, the intestinal propulsion rate of mice was significantly lower than that of the blank group (P<0.05); 14="" while="" the="" intestinal="" propulsion="" rate="" of="" and="" days="" did="" not="" change="" significantly="" p="">0. 05).

　　Conclusion This study found that continuous intragastric gavage at a dose of 50 mg/kg for 7 days can significantly reduce the intestinal advancement rate and defecation volume of mice, and can create a relatively stable mouse model of colonic transit constipation. The exploration of the model of slow transit constipation induced by loperamide hydrochloride provides valuable data.